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SingleCAnalyzer is a free cloud platform for the analysis of single-cell RNA-seq data. It provides a full analysis from sequence files (FASTQ) with the application of state-of-the art analysis methods.

Our analysis pipeline applies a confident protocol which avoids the need for configuration using complex parameters. It performs a quick and semiautomated analysis which does not require the installation of software on your computer.

Self-exploratory and Interactive Results

Analysis Pipeline

The analysis pipeline includes:

  • Demultiplexing
  • Alignment of FASTQ files
  • Read trimming
  • Quality control of samples
  • Feature selection
  • Cell classification
  • Dimensional reduction
  • Unsupervised clustering of samples
  • Expression comparison between groups
  • Functional enrichment of differential genes
  • Gene set expression analyses (GSEA)

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